Behaviour 2019
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Anxiety-like response and gene expression in three zebrafish populations exposed to alcohol.
Thais Agues-Barbosa1, Francisco C. da-Silva-Junior2, Jackson N. G. Lima1, Silvia R. Batistuzzo-Medeiros2, Ana C. Luchiari1. 1Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal, Outside US/Canada, Brazil; 2Department of Cell Biology and Genetics, Federal University of Rio Grande do Norte, Natal, Outside US/Canada, Brazil

The variations in the effects of alcohol between individuals of the same population may be related to the differences in the genetic background. In this study, we investigated anxiety-like responses and gene expression of three zebrafish populations (AB, TU, and WT n=180) exposed to a 2x2 alcohol protocol (0% or 0.50% chronic doses combined to 0% or 0.5% acute doses). We analyzed anxiety through a novel tank test, in addition to the expression of TPH1, ADA1, and BDNF genes through qPCR. Anxiety was increased in AB and TU fish during withdrawal and decreased in AB acute alcohol. Levels of TPH1 and ADA1 were similar between AB and TU fish (increasing in both acute and withdrawal treatments), while WT fish exhibited no significant response. BNDF has diminished in AB and WT fish from withdrawal, while TU fish showed increased levels in chronic and withdrawal. Our results suggest that zebrafish populations feature differences in response to alcohol. Fish from mixed genetic backgrounds produced unexpected results, supporting inbred populations are more indicated to translational research due to their known genetic background and, therefore providing data reliability.