Presentation Details
| Acute Stress Disrupts Brain Redox Homeostasis in a Cichlid Fish Farjana A.Chamily, Sarah H.Wojtylko, Peter D.Dijkstra. Central Michigan University, Mount Pleasant, MI, USA |
Abstract
Stress increases metabolic activity in the brain, elevating the production of reactive oxygen species, which are typically buffered by antioxidant systems to maintain redox homeostasis. However, how animals cope with acute oxidative insults while preserving redox balance is relatively unknown. Using Astatotilapia burtoni, we examined temporal changes in brain oxidative status following an acute stressor. Individually housed males were exposed to 3 minutes of air exposure and sampled at 15-, 30-, and 60-minutes post-stress. Acute stress did not affect malondialdehyde (MDA, a marker of lipid peroxidation) or superoxide dismutase (SOD, antioxidant enzyme) at any time point. However, antioxidant capacity, measured as the ratio of reduced to oxidized glutathione, showed a significant interaction between treatment and time, with air exposed males showing increased oxidative stress at 60 minutes post-stress. Interestingly, stressed males with high gonadosomatic index (GSI) had significantly lower MDA and higher SOD. These findings indicate that stress disrupts brain redox homeostasis, but individuals with high GSI can regulate oxidative defenses to maintain redox balance.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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