Presentation Details
| Using Behavior to Guide Isoflurane Anesthesia across Sex and Life Stage in Madagascar Hissing Cockroaches Charlie R.Schmidt, Samuel O.Durosaro, Meghan R.Barrett. Department of Biology, Indiana University Indianapolis, Indianapolis, IN, USA |
Abstract
Veterinarians now recommend isoflurane as a more humane anesthetic for insects undergoing invasive procedures; however, few guidelines exist for isoflurane anesthesia with diverse insects. The Madagascar hissing cockroach (Gromphadorhina portentosa) is frequently used in invasive procedures, including limb removal and electrode implantation, in research and pedagogical contexts. While prior studies have developed some guidance using continuous-flow anesthesia delivery systems, this method is impractical for many researchers and instructors with limited access to expensive equipment. Here, we develop a version of the drop method, used in rodent research when continuous-flow systems are unavailable, that is cost-effective and easy to implement. We tested adult and juvenile G. portentosa at isoflurane concentrations from 11.25 - 22.5%, using behavioral measures to assess knockdown and recovery time, potential stress on induction, and effective concentrations for reliable immobilization. Ultimately, we establish practical guidelines for isoflurane use with the drop method and describe standard behavioral measures of induction and recovery aimed at improving welfare for this species.
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No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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