Presentation Details
| Visual learning using optogenetics in freely walking Drosophila Shubham Rathore1, Edward M.Rogers1, Carmen Morrow1, Frank Loesche1, Peter Polidoro1, Will Dickson2, Michael B.Reiser1. 1Janelia Research Campus, HHMI, Ashburn, VA, USA.2IO Rodeo, Pasadena CA, USA |
Abstract
Many animals use vision for a range of navigation behaviors, relying on diverse features of their environments. In Drosophila melanogaster, numerous studies have demonstrated that flies can associate visual cues with reward or punishment. However, these experiments often require complex setups and specialized knowledge, hindering replicability of these fascinating behaviors. We are developing a modular setup that incorporates features from multiple visual learning paradigms to standardize these assays.
We drive avoidance behaviors in flies, using transgenic lines expressing an optogenetic depolarizer in specific heat-sensing neurons to investigate : (1) object learning - discrete visual features are paired with reinforcement in opposing quadrants; and (2) visual place learning - single safe zone--relief from punishment--is paired with a consistent feature of a visual panorama.
Given recent advances in the Drosophila neurobiology toolkit, we hope this platform, enabling reliable, replicable visual learning experiments, will provide a foundation for understanding neural circuits linking visual processing with experience-dependent behavior.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
We drive avoidance behaviors in flies, using transgenic lines expressing an optogenetic depolarizer in specific heat-sensing neurons to investigate : (1) object learning - discrete visual features are paired with reinforcement in opposing quadrants; and (2) visual place learning - single safe zone--relief from punishment--is paired with a consistent feature of a visual panorama.
Given recent advances in the Drosophila neurobiology toolkit, we hope this platform, enabling reliable, replicable visual learning experiments, will provide a foundation for understanding neural circuits linking visual processing with experience-dependent behavior.
No part of this publication may be reproduced, distributed, or transmitted in any form or by any means, including photocopying, recording, or other electronic or mechanical methods, without the prior written permission of the author.
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